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Figure 1.

Ten termite mandibles displaying Mn concentrated along the cutting edge.

More intense colour indicates higher concentration (Maximum of 2–3% as measured by SEM EDX). Mandibles are orientated with the teeth projecting towards the left of image.

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Table 1.

Elemental content of Tumulitermes tumuli Malpighian tubule (SEM EDX), soil, nest material and food source (ICP-MS/OES) in ppm (mean (SE)).

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Figure 2.

Composite PIXE image of a worker termite (Tumulitermes tumuli) showing distribution of 5 metals.

More intense colours indicate higher concentrations of metals. Iron appears concentrated around the antennae and lower abdomen; Zn and Ca in the Malpighian tubules with a weak accumulation over the entire body of Zn and Mn within Malpighian tubules and mandibles.

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Figure 3.

Excited X-ray spectra.

A: Whole termite body PIXE scan. B: SEM scan of the Malpighian tubule.

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Figure 4.

EDX maps of Malpighian tubules in association with the mid gut.

More intense colour indicates higher concentrations. Calcium, Mg, P and Zn are delineated within Malpighian tubules. Potassium is associated with fat bodies lying adjacent to the Malpighian tubules.

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Figure 5.

Synchrotron XFM images of Malpighian tubule concretions.

Scan size 8.0×8.0 µm2, 80×80 pixels, 1 s dwell per pixel. A: Composite image map of Zn and Ca (red and green). B: Zn map (red), C: Ca map (green). Image A demonstrates that high concentrations of Zn and Ca occur in mutually exclusive concretions.

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Figure 6.

SEM image of a stored food sphere.

Sphere consists of masticated grass, mulga leaves and other surface material.

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