Lungfish J chain molecular analysis.
A) Alignment of the J chain amino acid sequences of lungfish (JX999962), mouse (AAH06026), human (ABI63362), chicken (BAB83927), Xenopus (AAC05636), bullfrog (ACO52584), coelacanth (ENSLACP00000023629) and shark (AAO24897). “SS” indicates the interchain disulfide bridges and “TP” marks the Cys paired with the secretory tail of IgA or IgM. The conserved N-linked glycosilation site is indicated by “+”. Numbers (1–8) indicate the Cys residues in human sequences. “*” Indicates identical amino acids between all sequences, whilst “.” and “:” show conservative substitutions. Regions 1–3 were identified as in Braathen et al (2007) B) Conservation of Cys residues between lungfish and other vertebrates. C) Phylogenetic tree showing the evolutionary relationship of J chain molecules in vertebrates. The tree was constructed using the Neighbour-Joining method in MEGA 4 and was bootstrapped 10,000 times. GenBank accession numbers are as follows: lungfish (JX999962), mouse (AAH06026), human (ABI63362), chicken (BAB83927), Xenopus (AAC05636), bullfrog (ACO52584), coelacanth (ENSLACP00000023629) and shark (AAO24897).
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