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FTIR spectro-imaging of collagen scaffold formation during glioma tumor development

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Abstract

Evidence has recently emerged that solid and diffuse tumors produce a specific extracellular matrix (ECM) for division and diffusion, also developing a specific interface with microvasculature. This ECM is mainly composed of collagens and their scaffolding appears to drive tumor growth. Although collagens are not easily analyzable by UV-fluorescence means, FTIR imaging has appeared as a valuable tool to characterize collagen contents in tissues, specially the brain, where ECM is normally devoid of collagen proteins. Here, we used FTIR imaging to characterize collagen content changes in growing glioma tumors. We could determine that C6-derived solid tumors presented high content of triple helix after 8–11 days of growth (typical of collagen fibrils formation; 8/8 tumor samples; 91 % of total variance), and further turned to larger α-helix (days 12–15; 9/10 of tumors; 94 % of variance) and β-turns (day 18–21; 7/8 tumors; 97 % of variance) contents, which suggest the incorporation of non-fibrillar collagen types in ECM, a sign of more and more organized collagen scaffold along tumor progression. The growth of tumors was also associated to the level of collagen produced (P < 0.05). This study thus confirms that collagen scaffolding is a major event accompanying the angiogenic shift and faster tumor growth in solid glioma phenotypes.

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Acknowledgments

The authors are indebted to the “Ligue Nationale contre le cancer” and the “Agence Nationale de la Recherche” (ANR contract no bl-inter09_464249-MIAG-X) for their financial support.

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Authors and Affiliations

  1. Department of Chemistry, Government College University, 38000, Faisalabad, Pakistan

    Razia Noreen

  2. Academia Sinica, NANO-X Laboratory, No 128, Sec. 1, Academia Rd. Nankang, Taipei, 115-29, Taiwan, Republic of China

    Chia-Chi Chien & Yeukuang Hwu

  3. Inserm U1029 LAMC, Avenue des facultés, University of Bordeaux, Bat B2, 33405, Talence, France

    Hsiang-Hsin Chen, Vladimir Bobroff, Sophie Javerzat & Cyril Petibois

  4. Institut de Biochimie et Génétique Cellulaires, CNRS UMR5095, University of Bordeaux, 1, rue Camille Saint Saëns, CS 61390, 33077, Bordeaux Cedex, France

    Michel Moenner

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  1. Razia Noreen
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  2. Chia-Chi Chien
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  3. Hsiang-Hsin Chen
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  4. Vladimir Bobroff
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  5. Michel Moenner
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  6. Sophie Javerzat
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  7. Yeukuang Hwu
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  8. Cyril Petibois
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Corresponding author

Correspondence to Cyril Petibois.

Additional information

Published in the topical collection Morpho-Spectral Imaging with guest editors Cyril Petibois and Yeukuang Hwu.

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Noreen, R., Chien, CC., Chen, HH. et al. FTIR spectro-imaging of collagen scaffold formation during glioma tumor development. Anal Bioanal Chem 405, 8729–8736 (2013). https://doi.org/10.1007/s00216-013-7337-8

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  • DOI: https://doi.org/10.1007/s00216-013-7337-8

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