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Physicochemical stability and antibacterial mechanism of theabrownins prepared from tea polyphenols catalyzed by polyphenol oxidase and peroxidase

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Abstract

Tea polyphenols were used as substrates and oxidized successively by polyphenol oxidase and peroxidase to prepare theabrownins (TBs-dE). The conversion rate of catechins to TBs-dE was 90.91%. The ultraviolet and infrared spectroscopic properties and zeta potential of TBs-dE were characterized. TBs-dE is more stable at pH 5.0–7.0, about 25 °C or in dark environment. Ultraviolet light and sunlight can deepen its color due to the further oxidative polymerization. Mg2+, Cu2+, and Al3+ had a significant effect on the stability of TBs-dE. The inhibitory rates of TBs-dE (1 mg/mL) against Staphylococcus aureus and Escherichia coli DH5α were 51.45% and 45.05%, respectively. After TBs-dE treatment, the cell morphology of both bacteria changed, some cell walls were blurred, and the cytoplasmic content leaked. The research results can provide theoretical support for the industrialization of theabrownins.

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Acknowledgements

This research was financially supported by the National Natural Science Foundation of China (31871813).

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Authors and Affiliations

  1. Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan, 430068, China

    Xiaoqiang Chen, Tingting Chen, Jiayan Liu & Yan’an Wei

  2. National Center for Tea Quality Inspection and Testing, Hangzhou Tea Research Institute, All China Federation of Supply and Marketing Cooperatives, Hangzhou, 310016, China

    Weilong Zhou

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  1. Xiaoqiang Chen
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  2. Tingting Chen
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  3. Jiayan Liu
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  4. Yan’an Wei
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Correspondence to Xiaoqiang Chen.

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Chen, X., Chen, T., Liu, J. et al. Physicochemical stability and antibacterial mechanism of theabrownins prepared from tea polyphenols catalyzed by polyphenol oxidase and peroxidase. Food Sci Biotechnol 33, 47–61 (2024). https://doi.org/10.1007/s10068-023-01341-x

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