Click on image or enlarge button to enlarge
p-ERK Antibody (E-4): sc-7383
- p-ERK Antibody (E-4) is a mouse monoclonal IgG2a κ, cited in 3,217 publications, provided at 200 µg/ml
- p-ERK Antibody (E-4) is recommended for detection of ERK 1 phosphorylated at Tyr 204 and correspondingly phosphorylated ERK 2 of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-p-ERK Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to biotin for WB, IHC(P) and ELISA; and to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-ERK (E-4): sc-7383.
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for p-ERK Antibody (E-4) for WB and IHC(P) applications. These reagents are now offered in bundles with p-ERK Antibody (E-4) (see ordering information below).
p-ERK Antibody (E-4) is an IgG2a κ mouse monoclonal p-ERK antibody (also designated p-ERK antibody or p44/42 MAPK antibody) that detects ERK 1 phosphorylated at Tyr 204 and correspondingly phosphorylated ERK 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. p-ERK Antibody (E-4) is available as the non-conjugated anti-p-ERK antibody form, as well as multiple conjugated forms of anti-p-ERK antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ. | Haycock, JW., et al. 1992. Proc Natl Acad Sci U S A. 89: 2365-9. PMID: 1347949
- Purification of a murine protein-tyrosine/threonine kinase that phosphorylates and activates the Erk-1 gene product: relationship to the fission yeast byr1 gene product. | Crews, CM. and Erikson, RL. 1992. Proc Natl Acad Sci U S A. 89: 8205-9. PMID: 1381507
- The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. | Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
- Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase. | Boulton, TG., et al. 1991. Biochemistry. 30: 278-86. PMID: 1846291
- Identification of the regulatory phosphorylation sites in pp42/mitogen-activated protein kinase (MAP kinase). | Payne, DM., et al. 1991. EMBO J. 10: 885-92. PMID: 1849075
- ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF. | Boulton, TG., et al. 1991. Cell. 65: 663-75. PMID: 2032290
- Components of a new human protein kinase signal transduction pathway. | Zhou, G., et al. 1995. J Biol Chem. 270: 12665-9. PMID: 7759517
- ERK6, a mitogen-activated protein kinase involved in C2C12 myoblast differentiation. | Lechner, C., et al. 1996. Proc Natl Acad Sci U S A. 93: 4355-9. PMID: 8633070