TEGUH TRIYONO
Dept. of Clinical Pathology
Faculty of Medicine, Universitas Gadjah Mada
RSUP Dr. Sardjito
Yogyakarta
Pra-Transfusi
• Penggolongan darah
• Skrining antibodi
• Crossmatch
Apa itu crossmatch?
Crossmatch
1. Immediate Spin Crossmatch
3. Electronic Crossmatch
Metode
o Tube test
o Collumn Agglutination Test
o Microplate
o Solid Phase Red Cell Agglutination
MAYOR MINOR AUTOKONTROL
AGLUTINASI
KOMPATIBEL INKOMPATIBEL
Penyebab inkompatibel
Faktor lain?
• Golongan darah donor berbeda
dengan pasien
• Antibodi subgrup: misal Anti-A1
• Anti-A or anti-B dari ibu
• Rouleaux formation
• DCT donor positif
• Anti CD38 dalam serum
• Antibody of undetermined
specificity
• Contamination
Transfusi darah inkompatibel
Konsekuensi?
Transfusi pasien A RhD pos dengan
donor WB O Rh pos
Intravascular haemolysis of 51Cr-labelled ABO-incompatible red cells
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2
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