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Zellersatztherapie am Herzen: Fiktion oder reale Möglichkeit

Eine tierexperimentelle Annäherung

Cell replacement therapy in the heart: fiction or a real possibility

An animal experiment approach

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Zeitschrift für Herz-,Thorax- und Gefäßchirurgie Aims and scope

Zusammenfassung

Aufbauend auf ein murines Kryoinfarktmodell mit reproduzierbarer Infarktgröße, verlässlicher Einschränkung der linksventrikulären Myokardfunktion und niedriger primärer Sterblichkeit konnte der Einfluss der Transplantation verschiedenster Progenitor- und Stammzellen auf linksventrikuläre Kontraktilität, elektrische Vulnerabilität und postoperatives Überleben getestet werden. In die myokardiale Läsion implantierte, embryonale Kardiomyozyten intergrieren in den Gewebsverband, differenzieren in einen adulten Phänotyp und verbessern die Myokardfunktion. Diese Connexin 43 exprimierenden, kontraktilen Zellen koppeln elektrisch an das Wirtsmyokard, verbessern die elektrische Leitfähigkeit und schützen vor Post-Infarkt-Arrhythmien.

Abstract

Transmural cryolesions of a defined size were generated on the free left ventricular wall in mice. Using this approach, reproducible myocardial infarctions with a predictable decrease in myocardial function were generated. The potency of cellular cardiomyoplasty, employing different stem and progenitor cells, to improve contractility and impair electrical vulnerability was investigated. Intramyocardially injected fetal cardiomyocytes engrafted, showed further differentiation, increased left ventricular function and improved mid-term survival of the animals. Connexin 43 expressing cells coupled electrically to the host myocardium and reduced inducibility of ventricular arrhythmias.

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Interessenkonflikt

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Authors and Affiliations

  1. Klinik und Poliklinik für Herzchirurgie, Rheinische Friedrich-Wilhelms-Universität Bonn, Bonn, BRD, Sigmund-Freud-Str. 25, 53105, Bonn, Deutschland

    W. Röll, T. Bostani & A. Welz

  2. Institut für Physiologie I, Rheinische Friedrich-Wilhelms-Universität Bonn, Bonn, BRD, Bonn, Deutschland

    P. Sasse, M. Breitbach, D. Wenzel, A.M. Klein & B.K. Fleischmann

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  1. W. Röll
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  2. P. Sasse
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  3. M. Breitbach
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  4. D. Wenzel
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  5. A.M. Klein
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  6. T. Bostani
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Correspondence to W. Röll.

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Röll, W., Sasse, P., Breitbach, M. et al. Zellersatztherapie am Herzen: Fiktion oder reale Möglichkeit. Z Herz- Thorax- Gefäßchir 23, 170–176 (2009). https://doi.org/10.1007/s00398-009-0719-8

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  • DOI: https://doi.org/10.1007/s00398-009-0719-8

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