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Fluorochromes Suitable for Antibody Conjugation

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Abstract

This chapter is dedicated to fluorochromes used in conjugating antibodies or other proteins. The chapter classifies this group of fluorochromes according to their structural characteristics. It divides them into (1) large protein molecules (PE, APC, PerCP, et cetera), (2) small organic molecules (FITC, TRITC, Alexa molecules, et cetera), (3) polymeric molecules (Brilliant Violet, Pdots, et cetera), and (4) and nanocrystals (Qdots and Upconverting Nanoparticles). A fifth group is added, encompassing the tandem fluorochromes, classified based on their main excitation line. This group is thoroughly described, with special attention to the general principles underlying their functioning, and the issues they often present during multicolor analyzes are carefully described. This chapter mentions more than 200 molecules, providing—when available—information on their molecular structure, spectral behavior, use in flow and imaging techniques, and further information on unexpected behaviors and the possibility of artifacts.

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  1. University of Urbino, Urbino, Italy

    Claudio Ortolani

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Ortolani, C. (2022). Fluorochromes Suitable for Antibody Conjugation. In: Flow Cytometry Today. Springer, Cham. https://doi.org/10.1007/978-3-031-10836-5_15

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