. Anatomischer Anzeiger. Anatomy, Comparative; Anatomy, Comparative. 84 taken place, very thin free hand sHces are cut with a sharp razor from different parts of the head, tail and body of the pancreas re- spectively. Pieces from each of these sections are rapidly teased on the slide in Einger's solution and left exposed to the air without a cover glass while counting other slides. When a teased prepara- tion appears a little too blue, it can be improved by placing on a cover glass and setting aside while counting others. A certain amount of reduction usually takes place in the covered prepara

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. Anatomischer Anzeiger. Anatomy, Comparative; Anatomy, Comparative. 84 taken place, very thin free hand sHces are cut with a sharp razor from different parts of the head, tail and body of the pancreas re- spectively. Pieces from each of these sections are rapidly teased on the slide in Einger's solution and left exposed to the air without a cover glass while counting other slides. When a teased prepara- tion appears a little too blue, it can be improved by placing on a cover glass and setting aside while counting others. A certain amount of reduction usually takes place in the covered preparation. For a longer preservation of the contrast between islands and acini, small. Fig. 1. Islands of Langerhans in a teased preparation from subject 7. Sixteen islands of various sizes are shown in a single field of the microscope in sharp con- trast to the acinous tissue. Intra vitam staining with janus green, X 78. pieces of pancreas may be teased in ammonium molybdate (5% so- lution in water), instead of in Ringer's solution, if one is sure that the preparation shows the proper amount of reduction. However, I have found that ammonium molybdate soon clouds the preparation and renders a blue one valueless It sometimes happens that the reduc- tion has gone so far that a green stain in many of the islets no longer obtains. Careful teasing and study in this case will still show some. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Anatomische Gesellschaft. Jena : G. Fischer